What special attention should be paid when cultivating nitrifying bacteria at lower temperatures (1 to 3 degrees Celsius)?

When cultivating nitrifying bacteria at lower temperatures (1 to 3 degrees Celsius), special attention should be paid to the following points:

1. Provide sufficient oxygen: Nitrobacteria are aerobic microorganisms that require sufficient oxygen for metabolism and growth. At low temperatures, the oxygen solubility in water may decrease, so it is necessary to increase the oxygen supply to ensure sufficient oxygen supply for nitrifying bacteria. Oxygen solubility can be improved by increasing water agitation or using oxygen boosting equipment.
2. Provide appropriate nutrients: Nitrobacteria require ammonia nitrogen as an energy and carbon source. At low temperatures, the solubility of ammonia nitrogen may decrease, so it is necessary to increase the supply of ammonia nitrogen to support the growth of nitrifying bacteria. Ammonia nitrogen can be provided by adding ammonia compounds or organic compounds. In addition, nitrifying bacteria also require trace elements such as phosphorus and potassium to maintain their growth.
3. Maintain stable environmental conditions: At low temperatures, the chemical and physical properties of water may change, such as an increase in water density and a decrease in dissolved oxygen. Therefore, it is necessary to regularly monitor water quality parameters, such as pH value, dissolved oxygen, ammonia nitrogen concentration, etc., and take necessary measures to maintain stable environmental conditions.
4. Consider using nitrifying bacteria culture media: Under low-temperature conditions, it is possible to consider using nitrifying bacteria culture media specifically designed for low-temperature environments. These culture media typically contain low-temperature adapted strains of nitrifying bacteria that can more effectively carry out nitrification at low temperatures.
Through the above measures, the efficiency and success rate of cultivating nitrifying bacteria at low temperatures can be improved to a certain extent. However, it should be noted that under extreme low temperature conditions, the activity of nitrifying bacteria may be severely affected, so careful consideration should be given when selecting and adjusting culture conditions.